Journal: Methods in Molecular Biology

Article Title: Molecular Dermatology

doi: 10.1007/978-1-62703-227-8

Figure Lengend Snippet: Fig. 3. Immunopathological features of diseased mice. Direct immuno fl uorescence micros- copy of the skin from a mouse intraperitoneally injected with IgG from a BP patient. Note the linear deposition of human IgG (a, arrows ) and mouse C3 (b, arrowheads ) at the der- mal-epidermal junction. Scale bar: 200 m m.

Article Snippet: Fluorescent dye-conjugated antibodies: AF488-labeled polyclonal goat antibody speci fi c for mouse IgG and C3 (Cappel).

Techniques: Injection

Journal: Methods in Molecular Biology

Article Title: Molecular Dermatology

doi: 10.1007/978-1-62703-227-8

Figure Lengend Snippet: Fig. 5. Demonstration of tissue-bound and circulating collagen VII-speci fi c autoantibodies and histologic analysis. By indi- rect immuno fl uorescence (IF) microscopy ( a ) preimmune SJL mouse serum does not show binding to murine skin. In contrast, IgG autoantibodies in sera from SJL mice immunized with collagen are revealed by both ( b ) ELISA (data as mean of OD 490 nm ± SD) and recombinant His-mCVII-Cr and ( c ) indirect IF microscopy using normal mouse skin (magni fi cation, × 200). Direct immuno fl uorescence (IF) microscopy reveals linear deposition of ( d ) mouse IgG and ( e ) mouse C3 at the epidermal basement membrane (magni fi cation, ×200). Histological examination of a skin biopsy from the diseased SJL mouse shows ( f ) extensive subepidermal cleavage and neutrophil in fi ltration (magni fi cation, ×200).

Article Snippet: Fluorescent dye-conjugated antibodies: AF488-labeled polyclonal goat antibody speci fi c for mouse IgG and C3 (Cappel).

Techniques: Microscopy, Binding Assay, Enzyme-linked Immunosorbent Assay, Recombinant, Membrane